Prof Jane Mellor
My research group uses the budding yeast Saccharomyces cerevisiae and the nematode worm, Caenhorrhabitis elegans as model organisms in which to study how transcription, non-coding RNAs, higher order structures in chromatin, and histone modifications influence the response to changes in nutrient availability, metabolic state and ageing. Caloric restriction is known to increase lifespan in a wide range of organisms, including yeast, and may ameliorate the effects of age-related diseases but the mechanism involved are poorly understood.
Yeast and worms offer powerful tools for dissecting the pathways that allow cells to switch from active growth into a long-lived quiescent state. We use bioinformatics, 3C analysis, RNA-FISH, ChIP-seq, RNA-seq, nascent transcript mapping and most standard wet techniques. In addition, we produce models of cellular events such as gene expression and metabolic cycles in collaboration with physicists and mathematicians.
Mellor, J., Woloszczuk, R. & Howe, F.S. The Interleaved Genome. Trends Genet 32, 57-71 (2016).
Murray, S.C., Haenni, S., Howe, F.S., Fischl, H., Chocian, K., Nair, A. & Mellor, J. Sense and antisense transcription are associated with distinct chromatin architectures across genes. Nucleic Acids Res 43, 7823-37 (2015).
Nguyen T, Fischl H, Howe FS, Woloszczuk R, Serra Barros A, Xu Z, Brown D, Murray SC, Haenni S, Halstead JM, O'Connor L, Shipkovenska G, Steinmetz LM, Mellor J.
Transcription mediated insulation and interference direct gene cluster expression switches.
Elife. 2014 Nov 19;3:e03635. doi: 10.7554/eLife.03635. PMID:25407679
Howe FS, Boubriak I, Sale MJ, Nair A, Clynes D, Grijzenhout A, Murray SC, Woloszczuk R, Mellor J.
Lysine acetylation controls local protein conformation by influencing proline isomerization.
Mol Cell. 2014 Sep 4;55(5):733-44. doi: 10.1016/j.molcel.2014.07.004. Epub 2014 Aug 7. PMID:25127513
Murray SC, Serra Barros A, Brown DA, Dudek P, Ayling J, Mellor J.
A pre-initiation complex at the 3'-end of genes drives antisense transcription independent of divergent sense transcription.
Nucleic Acids Res. 2012 Mar;40(6):2432-44. doi: 10.1093/nar/gkr1121. Epub 2011 Nov 28. PMID:22123739